Technology

Pilot infrastructure for practical process development

Tenebrio molitor rearing requires coordination of the biological cycle, environmental conditions and daily operating procedures. Our pilot system supports colony management, rearing observation, procedure development and practical demonstrations for prospective users.

Tenebrio molitor larvae around a slice of vegetable
Larvae next to a moisture source in a rearing container, TeM incubator, October 2023.

Controlled conditions

Our infrastructure includes four climate-controlled chambers, sieving equipment and monitoring systems. It supports organised work with different developmental stages and rearing batches, alongside data collection for process improvement.

Illustration Schematic of a climate-controlled chamber with rearing trays and temperature and humidity monitoring.

From colony to product

Our development work covers breeding-colony maintenance, reproduction, rearing and larvae separation, as well as the assessment and testing of further processing.

  1. 1

    Breeding colonies

    Maintaining adult beetles and organising colonies.

  2. 2

    Reproduction

    Monitoring developmental stages and starting new batches.

  3. 3

    Larvae rearing

    Work in controlled conditions with record keeping.

  4. 4

    Separation

    Sieving and separating larvae from substrate and frass.

  5. 5

    Processing assessment

    Assessing application pathways and required analyses.

Illustration Tenebrio molitor life cycle: egg, larva, pupa and adult beetle.

Application pathways

Protein fractions, oil and frass represent different application pathways, each requiring suitable specifications, analysis and preparation for its intended use.

Protein fraction

Specification and analysis according to use.

Oil

Characterisation and preparation for a specific use.

Frass

Assessment of possible uses and required testing.

Transferring the process

Through TRISA, this knowledge was transferred to a partner in Ulcinj through pilot-space development, practical training and mentoring. The transfer addresses the whole operating process: facility organisation, colony handling, environmental monitoring and record keeping.

Illustration Knowledge transfer: from pilot infrastructure in Belgrade to the partner centre in Ulcinj.

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